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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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chromtech chiral-agp column  (Chrom Tech)
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral <t>AGP</t> <t>column</t> at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.
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Some representative chromatograms from the HPLC study on a chiral AGP column at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.

Journal: ACS Omega

Article Title: Characterization of the Interaction between Human α 1 ‑Acid Glycoprotein and Apremilast Enantiomers: HPLC, Isothermal Titration Calorimetry, Ultracentrifugation, and Docking Studies

doi: 10.1021/acsomega.5c07756

Figure Lengend Snippet: Some representative chromatograms from the HPLC study on a chiral AGP column at 25 °C. R and S denote the enantiomers of APR. (A) Eluent is 95% sodium phosphate buffer 5% IPA. (B) Eluent is 95% ammonium acetate buffer 5% IPA. (C) Eluent is 95% ammonium bicarbonate 5% IPA. Flow rate: 0.7 mL/min. The detection wavelength was set at 230 nm.

Article Snippet: The chiral AGP column (ChromTech Chiral-AGP) with dimensions of 150 mm × 4.0 mm was sourced from ChromTech Ltd. (Congleton, UK).

Techniques: